Flow cytometry analysis of prepared samples (DAPI staining method) to determine prepared larval catfish fish tissue karyotype. Cells are prepared using the CyStain® PI Absolute P reagent kit and modified protocol of Sysmex®. A pre-calibrated flow cytometer (BD FACSMelody, Bd Biosciences, San Jose, USA) must be used for determining ploidy. Larval batches must be separately calibrated with diploid control. About 58 hours required for flow cytometry measurements conducted by a trained flow cytometer operating expert. Flow cytometry laboratory must be NLA accredited.
Bids obtainable from: TumekaNC@daff.gov.za, Cost of Documents: No Cost, Payment Details: N/A Post or Deliver Bids to: Please forward all the required documents to TumekaNC@daff.gov.za.
For technical information please contact: Chris Fouche. Tel: (021) 4307054 Email: ChrisF@daff.gov.za. Hours: 07:00 - 15:30. For completion of bid documents please contact: Tumeka Ncobo, Tel: 021 402 3382, Email: TumekaNC@daff.gov.za, Hours: 07:30 - 16:00
